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Table 1 Metabolic profiles of E.coli mutants cultivated in NBS medium supplemented with 10 g/L glucose under anaerobic condition

From: High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli

Strains

Fermentation time (h)

CDW (g/L)

Glucose consumed (mM)

Succinate (mM)

Succinate yield (mol/mol)

Acetate (mM)

Pyruvate (mM)

Lactate (mM)

Specific growth rate during 0–24 h (g/g·h)

Specific glucose uptake rate during 0–24 h (g/g·h)

ZTK

42

1.03 ± 0.07

54.26 ± 0.26

54.91 ± 0.29

1.01 ± 0.006

26.10 ± 1.49

–

–

0.042 ± 0.001

0.44 ± 0.01

ZTK (pZY02E)

84

0.75 ± 0.03

53.33 ± 0.45

59.56 ± 0.40

1.12 ± 0.004

30.1 ± 0.03

–

–

0.021 ± 0.002

0.29 ± 0.01

ZTK (pZY02)

72

0.79 ± 0.01

54.26 ± 0.26

64.08 ± 0.40

1.18 ± 0.006

28.18 ± 0.18

–

–

0.021 ± 0.001

0.33 ± 0.03

WSA110

48

0.85 ± 0.01

53.89 ± 0.45

62.40 ± 0.38

1.16 ± 0.005

32.53 ± 0.62

1.50 ± 0.04

–

0.033 ± 0.0004

0.39 ± 0.03

WSA134

48

0.83 ± 0.003

54.26 ± 0.26

65.80 ± 0.22

1.21 ± 0.002

31.48 ± 0.57

–

–

0.029 ± 0.001

0.37 ± 0.006

WSA138

48

0.93 ± 0.008

54.44 ± 0.45

67.74 ± 0.36

1.24 ± 0.005

27.78 ± 0.56

–

–

0.035 ± 0.0008

0.43 ± 0.01

WSA146

48

0.89 ± 0.05

53.33 ± 0.45

69.68 ± 0.26

1.31 ± 0.005

23.68 ± 0.28

–

–

0.032 ± 0.0007

0.40 ± 0.01

WSA150

54

0.76 ± 0.008

51.48 ± 1.31

69.99 ± 1.55

1.36 ± 0.007

3.53 ± 0.04

1.44 ± 0.02

0.78 ± 0.06

0.031 ± 0.002

0.34 ± 0.03

WSA152

48

0.75 ± 0.009

50.93 ± 0.26

66.75 ± 0.35

1.31 ± 0.001

1.74 ± 0.04

3.49 ± 0.11

0.49 ± 0.05

0.023 ± 0.002

0.38 ± 0.02

WSA157

42

0.81 ± 0.014

51.67 ± 1.20

72.50 ± 1.59

1.40 ± 0.002

0.99 ± 0.03

1.71 ± 0.06

0.53 ± 0.09

0.032 ± 0.002

0.41 ± 0.01

WSA159

48

0.69 ± 0.005

52.59 ± 0.26

80.56 ± 0.10

1.53 ± 0.006

1.36 ± 0.14

1.43 ± 0.04

0.85 ± 0.07

0.027 ± 0.002

0.39 ± 0.03

WSA161

36

0.97 ± 0.02

50.56 ± 0.45

73.61 ± 0.70

1.46 ± 0.01

0.97 ± 0.17

0.96 ± 0.09

0.30 ± 0.03

0.051 ± 0.002

0.44 ± 0.03

WSA163

60

0.72 ± 0.05

52.50 ± 0.26

80.72 ± 0.24

1.54 ± 0.01

1.34 ± 0.03

1.51 ± 0.02

0.96 ± 0.02

0.024 ± 0.005

0.28 ± 0.03

WSA165

36

0.90 ± 0.03

52.59 ± 0.52

78.64 ± 0.79

1.50 ± 0.005

2.08 ± 0.05

2.00 ± 0.20

–

0.044 ± 0.002

0.44 ± 0.02

WSA167

36

1.03 ± 0.02

53.33 ± 0.45

77.82 ± 0.55

1.46 ± 0.008

1.87 ± 0.06

2.66 ± 0.14

–

0.054 ± 0.002

0.54 ± 0.005

  1. Bacteria were cultivated with 50 mL NBS medium in a 100 mL flask shaking at 220 rpm and 37 °C in anaerobic conditions with an initial OD600 of 0.6; “–” represents data were not measured in this work. Data are average values and standard deviations of triplicate experiments
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Microbial Cell Factories

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