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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Recombinant production of medium- to large-sized peptides in Escherichia coli using a cleavable self-aggregating tag

Fig. 4

Refolding and purification of insoluble peptides. The refolding and purification results of different peptides, designated with Leptin, CCL5, SDF1, and IGF1. Lane IC, insoluble fraction after intein-mediated cleavage; lane SR, soluble fraction after refolding of the insoluble peptides; lane PU, final product of target peptide after RP-HPLC purification; lanes 1, 2, and 3, quantification standards (Std) consisting of bovine serum albumin (BSA, 66.5 kDa) at 3, 1.5, and 0.75 µg/lane and aprotinin (6.5 kDa) at 1.5, 0.75, and 0.3 µg/lane, respectively. The molecular masses of the protein standards M1 and M2 are listed by the left and right side separately

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Microbial Cell Factories

ISSN: 1475-2859

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