Hydroxylation of diverse flavonoids by CYP450 BM3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities

Table 2 Comparison of ¹H-NMR and ¹³C-NMR of naringenin standard with hydroxylated naringenin (eriodictyol)

Carbon no.	Naringenin		Hydroxylated product (Eriodictyol)
Carbon no.	¹H NMR	¹³C NMR	¹H NMR	¹³C NMR
2	5.44 (dd, J = 12.8, 3.0 Hz)	78.90	5.38 (dd, J = 12.5, 3.1 Hz)	78.92
3-trans	3.27 (dd, J = 17.1, 12.8 Hz)	42.44	3.19 (dd, J = 17.1, 12.5 Hz)	42.54
3-cis	2.69 (dd, J = 17.1, 3.1 Hz)	42.44	2.68 (dd, J = 17.1, 3.2 Hz)	42.54
4		196.88		196.84
4a		102.24		102.26
5-OH	12.16 (s)	163.96	12.15 (s)	163.94
6	5.89 (s)	96.25	5.88 (m)	96.21
7-OH	10.78 (s)	167.11	10.80 (s, 1H)	167.10
8	5.89 (s)	95.43	5.88 (m)	95.41
8a		163.41		163.37
1′		129.32		129.91
2′	7.32 (m)	128.82	6.88 (s)	115.79
3′	6.80 (m)	115.63		146.18
3′-OH			9.05 (d, J = 34.2 Hz)
4′-OH	9.59 (s)	158.20	9.05 (d, J = 34.2 Hz)	145.65
5′	6.80 (m)	115.63	6.75 (s)	114.81
6′	7.32 (m)	128.82	6.75 (s)	118.41

ISSN: 1475-2859