Hydroxylation of diverse flavonoids by CYP450 BM3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities

Table 1 The conversion product, HPLC-PDA, HR-QTOF ESI/MS and UV maxima analyses of acceptor substrates in in vitro reaction using CYP450 BM3 and its variant proteins M13 and M15

Substrates				Products
Name	HPLC (t _R) min	Mass [M + H]⁺ m/z ⁺~	UV maxima (nm)	Name	% conversion M13	% conversion M15	HPLC (t _R) min	Mass [M + H]⁺ m/z ⁺~	UV maxima (nm)
Naringenin	16.429	273.0756	286	Hydroxylated	15.16	2.82	15.426	289.0710	287
Apigenin	16.241	271.0608	254, 336	Hydroxylated (a1)	4.89	1.14	15.274	287.0540	247, 339
3-HF	21.310	239.0702	235, 305	Hydroxylated (h1)	4.47	Trace	17.470	255.0734	241, 306, 339
Flavanone	21.645	225.0916	252, 318	Hydroxylated (f2)	12.26	Trace	16.355	241.0864	224
				Hydroxylated (f3)	8.87	Trace	16.798	241.0857	224
				Di-hydroxylated (f1)	9.65	5.69	11.461	257.0811	240; 278, 339
Genistein	16.622	271.0608	260	Hydroxylated (g1)	33.38	1.95	14.836	287.0552	260
Genistein	16.622	271.0608	260	Hydroxylated (g2)	2.77	1.02	15.485	287.0569	268
Daizein	15.264	255.0656	248	Hydroxylated (d1)	14.09	5.37	13.683	271.0607	254
Daizein	15.264	255.0656	248	Hydroxylated (d2)	4.81	1.67	14.369	271.0603	257
Biochanin A	19.444	285.0762	260	Hydroxylated (b3)	27.73		17.400	301.0711	267
				De-methylated (b2)	3.44	1.11	17.096	271.0600	260
				De-methylated plus hydroxylated (b1)	2.47	Trace	16.376	287.0556	268

ISSN: 1475-2859