Table 2 Primers used for plasmid construction
From: Heterologous expression of antigenic peptides in Bacillus subtilis biofilms
Amplified segment | Oligonucleotide sequences |
|---|---|
tasA-sipW-tasA operon(1) | Fwd: 5′- ATGCGGATCCTCAGAGTTAAATGGTATTGCT-3′ Rev: 5′-GCATTCTAGAATTTTTATCCTCGCTATGCGC |
tasA-sipW-tasA operon(2) | Fwd.: 5′-ATGCCTCGAGGGATCCTCAGAGTTAAATGGTATTGCT-3′ Rev.: 5′-GCATGCGGCCGCATTTTTATCCTCGCTATGCGC-3′ |
mCherry | Fwd.: 5′-GATCTCTAGA ATGGTGAGCAAGGGCGAGGAG-3′ Rev.: 5′-GATCGAGCTCGGATCC TTACTTGTACAGCTCGTCCAT-3′ |
(102-207)EgTrp | Fwd.: 5′ ATGCGCGGCCGCCGAAACATCTACTAAGCTTGAC-3′ Rev.: 5′ GATCCCCCGGGGGATCC TTACTCTTGCTCGGAGACTTCGAG-3′ |
(102-278)EgTrp | Fwd.: 5′ ATGCGCGGCCGCCGAAACATCTACTAAGCTTGAC-3′ Rev.: 5′ GATCCCCGGGGGATCC TCAGAAGGAAGTGAGCTCCGC-3′ |
(170-369)EgA31 | Fwd.: 5′ ATGCGCGGCCGCCGCAGCT5GAAAAACAAGCCATG-3′ Rev.: 5′ GATCCCCGGGGGATCC TCACCTTGTTTCAAGCATTTCAAT-3′ |
(370-583)EgA31 | Fwd.: 5′ ATGCGCGGCCGCCGCTGAGACTAAAGAAATTAAT-3′ Rev.: 5′ GATCCCCGGGGGATCC TCAATCTCTTTCGAGCTGTTTGAT-3′ |