Table 1 Bacillus subtilis strains used in this study
From: Heterologous expression of antigenic peptides in Bacillus subtilis biofilms
Strain | Genotype | Reference/source |
|---|---|---|
168 | Wild type domesticated | Kolter Lab., Harvard Medical School |
NCIB3610 | Wild type undomesticated | Kolter Lab., Harvard Medical School |
P tapA -yfp (CA018) | amyE::P tapA -yfp; Spc R | Vlamakis et al. [26] |
tasA (CA017) | tasA::Km R | Vlamakis et al. [26] |
sinR (DS92) | sinR::Spc R | Kearns et al. [46] |
tasA/sinR | tasA::Km R ; sinR::Spc R | This study |
TasA-mCherry(CA113) | amyE::yqxM-sipW-tasA-mCherry; Cm R | This study |
tasA/TasA-mCherry | tasA::Km R ; AmyE::yqxM-sipW-tasA-mCherry; Cm R | This study |
tasA/sinR/TasA-mCherry | tasA::Km R ; sinR::Spc R ; amyE::yqxM-sipW-tasA-mCherry; Cm R | This study |
tasA/sinR | tasA-sinR::Km R | This study |
tasA/sinR/TasA-(102-207)EgTrp | tasA-sinR::Km R ; amyE::yqxM-sipW-tasA-(102-207)EgTrp; Spc R | This study |
tasA/sinR/TasA-(102-278)EgTrp | tasA-sinR::Km R ; amyE::yqxM-sipW-tasA-(102-278)EgTrp; Spc R | This study |
tasA/sinR/TasA-(170-369)EgA31 | tasA-sinR::Km R ; amyE::yqxM-sipW-tasA-(170-369)EgA31; Spc R | This study |
tasA/sinR/TasA-(370-583)EgA31 | tasA-sinR::Km R ; amyE::yqxM-sipW-tasA-(370-583)EgA31; Spc R | This study |