Fig. 5
From: Heterologous expression of antigenic peptides in Bacillus subtilis biofilms
Wild type and recombinant spores display equal performances. Spores were tested under different conditions as: a heat resistance, b acidic medium and c shelf-life storage. Data represent the mean ± SEM from three independent experiments (t-test, not significant, p value >0.05). Error bars indicate SEM. d Transmission electron microscopy of wild type, tasA/sinR, TasA-(102-207)Egtrp, TasA-(102-278)EgTrp, TasA-(170-369)EgA31 and TasA-(370-583)EgA31 spore strains. Spores were frozen with liquid nitrogen, fixed with glutaraldehyde, counterstained and photographed. Black arrowhead, spore coats; white arrowhead, spore cortex peptidoglycan; star, spore protoplast. Scale bars are 50 and 100 nm, as indicated