Fig. 3

a Immunoblotting of biofilm extract at 72 h for wild type, tasA, sinR and tasA/sinR (single antibiotic selection) strain for the detection of TasA using an anti-TasA antibody. b Schematic representation of tapA operon carrying E. granulosus antigenic peptides, EgTrp and EgA31, fused in frame at the 3′ end of tasA. tapA, anchoring and assembly protein; sipW, signal peptidase and tasA, major protein matrix. The amino acid region corresponding to each antigenic peptide is indicated. For simplicity of the figures, TasA-(102-207)EgTrp, TasA-(102-278)EgTrp, TasA-(170-369)EgA31 and TasA-(370-583)EgA31 are named as (102-207)EgTrp, (102-278)EgTrp, (170-369)EgA31 and (370-583)EgA31, respectively. Diagram not to scale. c Top view of biofilm architecture from wild type, tasA/sinR, TasA-(102-207)EgTrp, TasA-(102-278)EgTrp, TasA-(170-369)EgA31 and TasA-(370-583)EgA31 strains at 72 h. Scale bar is 1 cm. d Immunoblotting of biofilm extract for wild type (lanes 1 and 5), tasA/sinR (lanes 2 and 6), TasA-(102-207) EgTrp (lanes 3 and 7) and TasA-(102-279)EgTrp (lanes 4 and 8) strains detected with anti-TasA (left panel) and anti-EgTrp (right panel) antibodies. The white arrowhead indicates the position of the TasA-EgTrp antigenic peptides and the red arrow indicates the position of TasA. e Immunoblotting of biofilm extracts for wild type (lanes 1 and 4), tasA/sinR (lanes 2 and 5), TasA-(170-369)EgA31 (lane 3) and TasA-(370-583)EgA31 (lane 6) strains detected with anti-TasA (upper panel) and anti-EgA31 (lower panel) antibodies. The white arrowhead indicates the position of the TasA-EgA31 antigenic peptides and the red arrow indicates the position of TasA. The protein molecular weights marker (kDa) is indicated