Fig. 1
From: Heterologous expression of antigenic peptides in Bacillus subtilis biofilms
Spatial distribution of TasA within the biofilm. Vertical thin sections of biofilms harboring a yfp reporter fusion to P tapA (a) or a TasA-mCherry fusion protein (b). Biofilms were frozen at 72 h of development prior to cryosectioning and fixation. Images represent partial colonies, the edge is shown at the left and the agar surface is at the bottom. Transmitted light images were overlaid with fluorescence images that were false-colored for each reporter. Bar 50 µm