Table 2 Escherichia coli strains and plasmids used in this study
From: Enhanced production of polyhydroxybutyrate by multiple dividing E. coli
Names | Descriptions | References |
|---|---|---|
Strains | ||
E.coli JM109 | endA1 recA1 gyrA96 thi-1 hsdR17 (r −k ,m +k ) relA1 supE44 D (lac-proAB) [F′traD36 proAB laqIqZΔM15] | Invitrogen Inc. |
E.coli JM109ΔminC | carrying minC gene knock out for mutagenesis | This study |
E.coli JM109ΔminD | carrying minD gene knock out for mutagenesis | This study |
E.coli JM109ΔminCD | carrying minC and minD genes knock out for mutagenesis | This study |
Plasmids | ||
pKD46ReA | λ-Red recombinase expression helper plasmid, oriR101, repA101(ts), AmpR | Datsenko and Wanner [45] |
pKD13 | Template plasmid with KanR gene and FLP recognition target | Datsenko and Wanner [45] |
pCP20 | FLP recombinase helper plasmid, ts-rep, AmpR, CmR | Datsenko and Wanner [45] |
p15a-Pbad-ftsQLWN | p15A ori, arabinose promoter inducible expression of ftsQ,-L,-W,-N genes, CmR | This study |
p15a-Pglta-ftsQLWN | p15A ori, glta promoter control the expression of ftsQ,-L,-W,-N genes, CmR | This study |
p15a-ftsZ | p15A ori, arabinose promoter inducible expression of ftsZ gene, CmR | This study |
p15a-blank | p15A ori, blank vector, KanR | This study |
ptk-mreB | pTKRED derivate constitutive expression endogenous mreB gene, KanR | Jiang et al. [35] |
ptk-mreB-ftsZ | pTKRED derivate constitutive expression endogenous mreB gene and ftsZ gene, KanR | This study |
ptk-blank | pTKRED derivate blank vector, KanR | Jiang et al. [35] |
pBBR1-MCS1 | Cloning vector, CmR | This study |
pBBR-Pbad-ftsQLWN | pBBR1-MCS1with arabinose promoter inducible expression ftsQ,-L,-W,-N genes, CmR | This study |
pBHR68 | A pBluescript II SK-derivative containing phbCAB operon from Ralstonia eutropha H16 with native promoter, Amp | Spiekermann et al. [46] |