The comprehensive profile of fermentation products during in situ CO2 recycling by Rubisco-based engineered Escherichia coli

Table 3 Fermentation profiles of E. coli MZLF derived strains

E. coli strains	Glucose consumption	Molar yield (mmol/mmol)								Carbon recovery (%)
E. coli strains	(mM)	Pyr	Ace	EtOH	Lac	Suc	For	CO₂	Biomass	Carbon recovery (%)
MZLF	59 ± 7	0.16 ± 0.12	0.66 ± 0.09	0.84 ± 0.14	0.04 ± 0.02	0	1.54 ± 0.28	0.02 ± 0.005	0.37 ± 0.05	92 ± 9
MZLF3 + IP	55 ± 1	0.46 ± 0.02	0.41 ± 0.00	0.87 ± 0.01	0.03 ± 0.00	0	1.30 ± 0.02	0.001 ± 0.00	0.54 ± 0.02	98 ± 1
MZLFB	58 ± 5	0.14 ± 0.03	0.67 ± 0.07	0.82 ± 0.10	0.04 ± 0.01	0	1.56 ± 0.19	0.01 ± 0.003	0.40 ± 0.03	92 ± 5
MZLFB + IP	50 ± 2	0.46 ± 0.04	0.40 ± 0.03	0.87 ± 0.07	0.03 ± 0.01	0	1.24 ± 0.11	0	0.52 ± 0.04	96 ± 7
MZLFB + ace + IP	51 ± 1	0.61 ± 0.06	0.25 ± 0.05	0.82 ± 0.04	0.02 ± 0.0	0	1.13 ± 0.06	0	0.50 ± 0.03	94 ± 4

The calculation of carbon recovery includes ethanol, acetate, formate, lactate, succinate, malate, CO₂, and biomass. The elementary biomass composition of CH_1.77O_0.49N_0.24 is used for the calculations of carbon recovery [18]
IP and ace represent the supplement of 0.05 mM IPTG and 30 mM acetate at 8 h during the cultivation
Pyr pyruvate, Ace acetate, EtOH ethanol, Lac lactate, Suc succinate, For formate

ISSN: 1475-2859