Table 1 List of bacterial strains and plasmids used in this study
Name | Descriptions | Reference |
|---|---|---|
Bacterial strains | ||
E. coli BL21 (DE3) | F-, dcm, ompT, gal, lon, hsdSB (rB−, mB−), λ (DE3[lacI, lacUV5-T7 gene 1, ind1, sam7,nin5]) | Lab stock |
J3 | E. coli BL21 (DE3) harboring rbcLS-pET30a + (M259T) | [15] |
JB | E. coli BL21 (DE3) harboring both PBAD-his6-prkA-pACYC184 and rbcLS-pET30a + (M259T) | [15] |
MZ | E. coli BL21 (DE3) Δzwf | [16] |
MZL | MZ, Δldh | This study |
MZLF | MZL, Δfrd | This study |
MZLF1 | MZLF harboring PBAD-his6-prkA-pACYC184 | This study |
MZLF3 | MZLF harboring rbcLS-pET30a + (M259T) | This study |
MZLFB | MZLF harboring both PBAD-his6-prkA-pACYC184 and rbcLS-pET30a + (M259T) | This study |
Plasmids | ||
pKD46 | araC, bla, oriR101, repA101 (Ts), araC-P araB -γ-β-exo (encode λ Red recombinases), temperature-conditional replicon | [30] |
pkD13 | bla, FRT-kan-FRT | [30] |
pCP20 | FLP +, λ cI857+,λ PR Pepts, bla, catF | [31] |
PBAD-his6-prkA-pA CYC184 | Recombinant plasmid carries prkA gene (derived from Synechococcus PCC7492) for the overexpresion of phosphoribulokinase (PrkA) under the control of PBAD | [13] |
rbcLS-pET30a + (M259T) | Recombinant plasmid carries engineered rbcLS gene (originated from Synechococcus PCC6301) for the overexpresion of engineered Rubisco (M259T) under the control of PT7 | [13] |
rbcLS-pET30a + (M259T, K198G) | Recombinant plasmid carries engineered rbcLS gene (originated from Synechococcus PCC6301) for the overexpresion of engineered Rubisco (M259T and K198G) under the control of PT7 | This study |
rbcLS-pET30a + (M259T, K198G, D200G, E201G) | Recombinant plasmid carries engineered rbcLS gene (originated from Synechococcus PCC6301) for the overexpresion of engineered Rubisco (M259T, K198G, D200G, and E201G) under the control of PT7 | This study |
rbcLS-pET30a + (M259T, K198G, K172G) | Recombinant plasmid carries engineered rbcLS gene (originated from Synechococcus PCC6301) for the overexpresion of engineered Rubisco (M259T, K198G, and K172G) under the control of PT7 | This study |
rbcLS-pET30a + (M259T, K198G, K331G) | Recombinant plasmid carries engineered rbcLS gene (originated from Synechococcus PCC6301) for the overexpresion of engineered Rubisco (M259T, K198G, and K331G) under the control of PT7 | This study |