Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger

Table 4 An overview of controlled batch cultivations with IA producing A. niger strains

Strain	Highest titer g/L		Max productivity g/L/h		Yield g/g glucose
Strain	Itaconate	Citrate	Itaconate	Citrate	Itaconate	Citrate	Biomass (g/kg)
AB1.13 CAD 4.1	1.45	15.95	0.01	0.19	0.02	0.22	17.33
AB1.13 CAD + MFS 3.9	1.36	4.19	0.02	0.09	0.04	0.11	9.54
AB1.13 + CAD + MFS + MTT #08	2.48	21.99	0.02	0.13	0.03	0.29	15.59
AB1.13 + CAD + MFS + MTT #63	9.52	18.03	0.05	0.11	0.13	0.24	13.53
AB1.13 + CAD + MFS + MTT #49	11.74	13.17	0.08	0.12	0.16	0.18	15.02
AB1.13 + CAD + MFS + MTT #49	12.96	8.91	0.15	0.13	0.20	0.14	21.69
AB1.13 CitB#77	17.02	1.96	0.16	0.02	0.28	0.03	9.18
AB1.13 CitB#101	11.69	4.92	0.11	0.07	0.19	0.08	15.13
AB1.13 CitB#99	26.22	0.30	0.35	NA	0.37	NA	9.9
AB1.13 CitB#113	23.42	0.35	0.26	NA	0.33	NA	12.38

Overview of titer, productivity and yield of IA and CA during controlled batch-cultivations. Yield and biomass are measured at point of highest titer. In Fig. 9 an overview of the metabolic pathway consisting of the activities encoded by cadA, citB, mttA and mfsA is given

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