Fig. 6

Osmostress protection by ectoine and 5-hydroxyectoine. Strains MC4100 (ProP⁺ ProU⁺), MKH13 (ProP⁻ ProU⁻), MKH17 (ProP⁺ ProU⁻) and BK32 (ProP⁻ ProU⁺) were grown either in MMA, or in MMA with 0.8 M NaCl in the absence or the presence of the osmostress protectants glycine betaine, ectoine, and 5-hydroxyectoine (provided at a final concentration of 1 mM) at 37 °C for 24 h. The obtained growth yield was determined by measuring the OD₅₇₈ of the cultures. The values shown are the means and standard deviations of two independently grown cultures