Primer name | Restriction enzyme | Oligonucleotide sequence (5′–3′)a | Function |
---|---|---|---|
pBIG-FMa | HindIII | CGGCCGAAGCTTATGGCTGCTGCTCCCAGTGTGAGGAC | MaFADS6-I amplification for binary plasmid construction |
pBIG-RMa | XhoI | CGGGGCCTCGAGTTACTGCGCCTTACCCATCTTGGAGG | |
pBIG-FMp | HindIII | TACCGAAGCTTATGTGCCCGCCGAAGACGGACGGC | MpFADS6 amplification for binary plasmid construction |
pBIG-RMp | StuI | TAAAAGGCCTTCAGTGCGCCTTCTCCGCCTTGCCG | |
FMa | – | ATGGCTGCTGCTCCCAGTGTGAGGAC | Target genes insert detection for binary plasmid construction |
RMa | – | TTACTGCGCCTTACCCATCTTGGAGG | |
FMp | – | ATGTGCCCGCCGAAGACGGACGGC | |
RMp | – | TCAGTGCGCCTTCTCCGCCTTGCCG | |
HisproF | – | CACACACAAACCTCTCTCCCACT | T-DNA insert detection for binary plasmid construction |
TrpCR | – | CAAATGAACGTATCTTATCGAGATCC | |
RT-MaD6-F | – | ATGGCTGCTGCTCCCAGTGTGAGGAC | qPCR for MaFADS6-I transcript level measurement |
RT-MaD6-R | – | CACCTTGTTGTCGATGATCATCAGGAAGGG | |
RT-MpD6-F | – | ATGTGCCCGCCGAAGACGGAC | qPCR for MpFADS6 transcript level measurement |
RT-MpD6-R | – | CGTTTTGAGATCGACGGGCGCG | |
18SRTF | – | CGTACTACCGATTGAATGGCTTAG | Internal control gene for qPCR |
18SRTR | – | CCTACGGAAACCTTGTTACGACT |