Application of a delta-6 desaturase with α-linolenic acid preference on eicosapentaenoic acid production in Mortierella alpina

Table 1 Primers used in this study

Primer name	Restriction enzyme	Oligonucleotide sequence (5′–3′)^a	Function
pBIG-FMa	HindIII	CGGCCGAAGCTTATGGCTGCTGCTCCCAGTGTGAGGAC	MaFADS6-I amplification for binary plasmid construction
pBIG-RMa	XhoI	CGGGGCCTCGAGTTACTGCGCCTTACCCATCTTGGAGG	MaFADS6-I amplification for binary plasmid construction
pBIG-FMp	HindIII	TACCGAAGCTTATGTGCCCGCCGAAGACGGACGGC	MpFADS6 amplification for binary plasmid construction
pBIG-RMp	StuI	TAAAAGGCCTTCAGTGCGCCTTCTCCGCCTTGCCG	MpFADS6 amplification for binary plasmid construction
FMa	–	ATGGCTGCTGCTCCCAGTGTGAGGAC	Target genes insert detection for binary plasmid construction
RMa	–	TTACTGCGCCTTACCCATCTTGGAGG
FMp	–	ATGTGCCCGCCGAAGACGGACGGC
RMp	–	TCAGTGCGCCTTCTCCGCCTTGCCG
HisproF	–	CACACACAAACCTCTCTCCCACT	T-DNA insert detection for binary plasmid construction
TrpCR	–	CAAATGAACGTATCTTATCGAGATCC	T-DNA insert detection for binary plasmid construction
RT-MaD6-F	–	ATGGCTGCTGCTCCCAGTGTGAGGAC	qPCR for MaFADS6-I transcript level measurement
RT-MaD6-R	–	CACCTTGTTGTCGATGATCATCAGGAAGGG	qPCR for MaFADS6-I transcript level measurement
RT-MpD6-F	–	ATGTGCCCGCCGAAGACGGAC	qPCR for MpFADS6 transcript level measurement
RT-MpD6-R	–	CGTTTTGAGATCGACGGGCGCG	qPCR for MpFADS6 transcript level measurement
18SRTF	–	CGTACTACCGATTGAATGGCTTAG	Internal control gene for qPCR
18SRTR	–	CCTACGGAAACCTTGTTACGACT	Internal control gene for qPCR

ISSN: 1475-2859