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Table 1 S. cerevisiae strains and plasmids used in this study

From: Lycopene overproduction in Saccharomyces cerevisiae through combining pathway engineering with host engineering

 

Description

Source

Strain name

 CEN.PK2-1C

MATa, ura3-52, trp1-289, leu2-3,112, his3Δ1, MAL2-8C, SUC2

EUROSCARF

 CEN.PK2-1D

MATα, ura3-52, trp1-289, leu2-3,112, his3Δ1, MAL2-8C, SUC2

EUROSCARF

 SyBE_Sc14C01

CEN.PK2-1C, Δgal80::HIS3

This study

 SyBE_Sc14C02

CEN.PK2-1C, Δgal1 Δgal7 Δgal10::HIS3

This study

 SyBE_Sc14C06

SyBE_Sc14C01, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C07

SyBE_Sc14C02, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C10

CEN.PK2-1C, Δgal1 Δgal7 Δgal10::HIS3, Δypl062w::KanMX

This study

 SyBE_Sc14C21

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C51

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C52

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C53

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C22

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C23

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 SyBE_Sc14C24

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 SyBE_Sc14C54

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 SyBE_Sc14C55

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 SyBE_Sc14C56

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 SyBE_Sc14C25

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 SyBE_Sc14C26

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 SyBE_Sc14C27

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 SyBE_Sc14C57

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 SyBE_Sc14C58

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 SyBE_Sc14C59

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 SyBE_Sc14C28

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 SyBE_Sc14C29

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 SyBE_Sc14C30

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 SyBE_Sc14C60

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 SyBE_Sc14C61

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 SyBE_Sc14C62

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 SyBE_Sc14C31

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 SyBE_Sc14C32

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 SyBE_Sc14C33

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C63

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C64

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C65

SyBE_Sc14C10, trp1::TRP1_T CYC1 -AaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C34

SyBE_Sc14C10, trp1::TRP1_T CYC1 -PaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C35

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C40

SyBE_Sc14C10, leu2::LEU2_T CYC1 -RFP

This study

 SyBE_Sc14C41

SyBE_Sc14C10, leu2::LEU2_T CYC1 -RFP-P GAL3

This study

 SyBE_Sc14C42

SyBE_Sc14C10, leu2::LEU2_T CYC1 -RFP-P GAL7

This study

 SyBE_Sc14C43

SyBE_Sc14C10, leu2::LEU2_T CYC1 -RFP-P GAL10

This study

 SyBE_Sc14C44

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T CYC1 -BtCrtI-P GAL3 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C45

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14C46

SyBE_Sc14C10, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1 , leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , ΔYGLCtau3::HphMX_P GAL7 -BtCrtI-T CYC1

This study

 SyBE_Sc14D04

CEN.PK2-1D, Δgal1 Δgal7 Δgal10::HIS3, Δypl062w::KanMX, trp1::TRP1_T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1

This study

 SyBE_Sc14D05

SyBE_Sc14D04, leu2::LEU2_T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14D06

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL3 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14D07

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 SyBE_Sc14D08

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , ΔYGLCtau3::HphMX_P GAL7 -BtCrtI-T CYC1

This study

 SyBE_Sc14D10

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , Δyjl064w::BleMX

This study

 SyBE_Sc14D11

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , Δrox1::BleMX

This study

 SyBE_Sc14D12

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , Δdos2::BleMX

This study

 SyBE_Sc14D13

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , Δrox1::BleMX, Δdos2::HphMX

This study

 SyBE_Sc14D14

SyBE_Sc14D04, leu2::LEU2_T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1 , Δrox1::BleMX, ΔYPRCdelta15::HphMX_P GAL1 -INO2-T CPS1

This study

Plasmid

 pJET1.2/blunt

Blunt-end PCR fragments cloning vector

Fermentas

 pCY01

pJET1.2/blunt possessing TRP1 homologous arm, T CYC1 -AaCrtI-P GAL10 -P GAL1 -AaCrtB-T PGK1

This study

 pCY02

pJET1.2/blunt possessing TRP1 homologous arm, T CYC1 -PaCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1

This study

 pCY03

pJET1.2/blunt possessing TRP1 homologous arm, T CYC1 -BtCrtI-P GAL10 -P GAL1 -PaCrtB-T PGK1

This study

 pCY04

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T ACT1 -tHMG1-P GAL10 -P GAL1 -PaCrtE-T GPM1

This study

 pCY05

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T ACT1 -tHMG1-P GAL10 -P GAL1 -SaCrtE-T GPM1

This study

 pCY06

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T ACT1 -tHMG1-P GAL10 -P GAL1 -AfCrtE-T GPM1

This study

 pCY07

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T ACT1 -tHMG1-P GAL10 -P GAL1 -BtCrtE-T GPM1

This study

 pCY08

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 pCY09

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T CYC1 -RFP

This study

 pCY10

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T CYC1 -RFP-P GAL3

This study

 pCY11

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T CYC1 -RFP-P GAL7

This study

 pCY12

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T CYC1 -RFP-P GAL10

This study

 pCY13

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T CYC1 -BtCrtI-P GAL3 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 pCY14

pJET1.2/blunt possessing LEU2 homologous arm with LEU2 marker, T CYC1 -BtCrtI-P GAL7 -T ACT1 -tHMG1-P GAL10 -P GAL1 -TmCrtE-T GPM1

This study

 pCY15

pJET1.2/blunt possessing YGLCtau3 homologous arm with HphMX marker, P GAL7 -BtCrtI-T CYC1

This study

 pCY40

pJET1.2/blunt possessing YPRCdelta15 homologous arm with HphMX marker, P GAL1 -INO2-T CPS1

This study