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Table 1 T. reesei genes used to engineer the ethanologenic yeast S. cerevisiae to a cellulose or hemicellulose degrader

From: Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei

Genes expresseda Enzyme localization Substrate Ethanol (g/l) YEtOH (% theory)b Reference
cel5a + bglA A. aculeatus Cell surface display (CSD) 45 g/l β-glucan 16.5 93 [160]
xyn2 + xylA A. oryzae CSD 100 g/l birchwood xylan 7.1 59 [161]
cel6a, cel5a + bglA A. aculeatus CSD 10 g/l phosphoric acid swollen cellulose (PASC) 2.9 89 [162]
cel7a Secretion PASC, bacterial microcrystalline cellulose [163]
cel6a, cel5a + bglA A. aculeatus CSD 20 g/l PASC 7.1 75 [164]
cel6a, cel5a + bglA A. aculeatus CSD 100 g/l pretreated rice straw 7.5 33 [164]
cel6a, cel7a + 20 other cellobiohydrolases Secretion 20 g/l avicel cellulose 3 33 [165]
cel6a, cel5a + bglA A. aculeatus  + swo1 CSD 20 g/l PASC 3.4 36 [166]
cel6a, cel5a + bglA A. aculeatus  + cdt1  N. crassa CSD 20 g/l PASC 4.3 45 [115]
cel6a, cel5a + bglA A. aculeatus  + commercial cellulase CSD 100 g/l avicel cellulose 48.9 87 [167]
cel6a, cel5a + bglA A. aculeatus  + commercial cellulase CSD 200 g/l rice straw 42.2 86 [167]
xyn2, xylA A. oryzae , bglA A. aculeatus CSD Slurry from rice straw pretreatment containing ~20 g/l poly-, oligo- and monomeric sugars 4.0 39 [151]
cel5a + cbh1 T. emersonii fused to CBM of cel7a + bglA A. aculeatus CSD and secretion 10 g/l PASC 2.6 and 2.9 87 and 82 [168]
  1. a Genes are derived from T. reesei or from the species given in subscript
  2. b Based on a stochiometry of 0.51 g ethanol per g consumed sugar. Residual sugars in non-hydrolyzed substrates were considered when the data were available