From: Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent
Solubilization agent
Refolding yield (%)
Overall recovery (%)
References
50 mM Tris, pH 8.5, 8 M urea
50
21
[14, 18]
50 mM Tris, pH 12, 2 M urea
86
42
[17, this study]
50 mM Tris, pH 8.5, 6 M n-propanol, 2 M urea
76
40
[14]
50 mM Tris, pH 8.5, 30 % TFE, 3 M urea
87
45
This study
