Table 1 Comparative analysis of solubilization efficiency and refolding yield of hGH from IBs while using high pH or TFE based buffer for solubilization
Solubilization buffer | Solubilized protein (mg) | Refolded protein (mg) | Solubilization efficiency (%) | Refolding yield (%) |
|---|---|---|---|---|
High pH buffer with 2 M urea and 1 mM DTT | 62.99 ± 3.92 | 34.45 ± 2.2 | 79.63 ± 4.96 | 54.89 ± 5.9 |
30 % TFE with 3 M urea and 1 mM DTT | 71.78 ± 2.94 | 61.43 ± 2.97 | 90.75 ± 3.72 | 85.59 ± 2.46 |