A new regulatory mechanism controlling carotenogenesis in the fungus Mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineering

Table 1 Mutants in carotenoid biosynthesis analysed in this work

Strain	Color in the dark	Color in the light	Mutation in carotenogenic genes
R7B (wild-type)	White	Yellow	None
MU206	Pale yellow	Yellow	None
MU212, 213, 214	White	White	5′ splice site intron 1 mcwc1c
MU215	White	White	5′ splice site intron 4 mcwc1c
MU216	White	Pale yellow	None
MU217	White	Pale yellow	None
MU218	Pale yellow	Deep yellow	None
MU260	White	White	D53G CarB
MU261	White	White	C436R CarRP
MU262	White	White	T308 M CarB
MU263	White	White	20 bp deletion carB
MU264	White	White	None
MU266	White	Pale yellow	S317F CarB
MU265, MU267, MU280	White	Pale yellow	K143E Mcwc1c
MU268, MU269, MU271, MU272	White	Pale yellow	S77P Mcwc1c
MU273, MU276,	White	White	G433D CarRP
MU274, MU275, MU277, MU284	White	White	C235R CarRP
MU278	White	White	Deletion in region carB-carRP
MU279^a	White	White	Deletion in region carB-carRP
MU281	White	White	S302F CarRP
MU282	White	Pale yellow	None
MU283	White	Pale yellow	None
MU285	White	Pale yellow	None
MU286^a	White	Pale yellow	None

^aMU279 and MU286 derived from UV-treated spores whereas the remaining mutants came from treatment with NTG

ISSN: 1475-2859