Table 3 Primers used in this study

oriT1-1S

CGAGAATTCGTAAGCGGAACGAAGTG

PCR amplification of bp 1–352, 65–282 and 188–282 of oriT1

oriT1-65S

CGAGAATTCTGTTAAAACGGTTACCGTTTTG

oriT1-352A

CAGGTCGACCTACATGAGCCACGATCAATC

oriT1-282A

CAGGTCGACTTTGGTCTAGCACGTTATACC

oriT1-188A

CAGGTCGACGACTTTGTCCGAACCTACAAG

oriT2-1S

TATGCATGCCAAAGGTACCGGACCGAACC

PCR amplification of bp 1–370, 156–370 and 273–370 of oriT2

oriT2-370A

CAGAAGCTTGAACGTGAGCCATGAGCGAG

oriT2-156S

TATGCATGCGAAGGGGGTCAAGGGGAATT

oriT2-273S

TATGCATGCATTTGGTATAACGTGCTAGAC

oriT1-24-S

TATGAATTCTTTGGTATAACGTGCTAGACCAAATCTGACGCTCAGTGG

pBMBT13, pBMBT16 and pBMBTmini

oriT2-24-A

CCCAAGCTTTATGGTCTAGCACGTTATACCAAAAAATGTCACTAATATT

MS

GAATTCGGATCCGAGCTCCTCGAGAGATCTTTTGGTCTAGCACGTTATACCAAAGTGTGTTCTTAATATTGTATG

pRec-mob1-Ts

MA

CGCGTCGACGCATGCGATTACAACAAGTCTATTCAG

SS

CGCGCATGCTCTGACGCTCAGTGGAAC

SA

GTCGACCCATGGGCTAGCGGTACCCGGGTTTGGTATAACGTGCTAGACCATACTATGCAAGGAACAATTTC

amyE-up-S

CGCGAATTCATGCGTTTCTCTCGGATAATTGG

BMB0260, BMB0261 and BMB0262

amyE-up-A

CTCGGATCCCTTCCGTCTAAATCAACTTC

amyE-down-S

CGCGTCGACGTTAACATCACCTGGGATTC

amyE-down-A

GCCAAGCTTTCATCTACGCTTCCTTTTAACTG

amyE-wS

GTGCCAATCCTGGTGTCATC

amyE-wA

GAAAATCCTCTTCCTAACCTG

5B-S

GGGTATACAAGAAGGTTGGG

cry5Ba detection

5B-A

CGTTTTCTGGTACAAGTTCC

2A-S

GCCGAATTCGATGTTGATTCTTAGAGCAATG

BMB0262

2A-A

GGCGAATTCGGTTAACTTGAAATGATTTCTCC

zmaJ-up-S

CGGGAATTCCATTGGTAGACCAGGATTTGC

BMB0263

zmaJ-up-A

CATGGATCCTCCACCTTCACGTAAAAGACG

zmaJ-down-S

CGGGGTACCGTACTTGCCTTATTAGGTTCCG

zmaJ-down-A

CACGTCGACACTTACACGAAGTGGTGGTG

zmaJ-wS

CAGCTGCTGATGTGTTTTTGG

zmaJ-wA

CTCAACTACCATAATAGTGTC

pKan-S

CCGGAATTCCATTTGAGGTGATAGGTAAG

pEMB0603-zmaJ

pKan-zmaJ-A

TATACATGTACTCATTTTAATAACCTCCTTTCTCTAGACCCCAAGAAGCTAATTATAAC

pKan-zmaJ-S

ATTAGCTTCTTGGGGTCTAGAGAAAGGAGGTTATTAAAATGAGTACATGTATACAAAAG

zmaJ-A

ACGCGTCGACTTATGAATCTTTACTGCTTTC