Table 2 Bacterial strains and plasmids used in this study

Bacillus thuringiensis strains

 AW48

Derivative of wild-type isolate HD73 cured of pHT73, containing pAW63::Tn5401, Tet^r

[36]

 BMB0260

Mutant of strain BMB171 in which the amyE gene is interrupted by in-frame deletion

This study

 BMB0261

Mutant of strain BMB171 in which the cry5Ba gene is inserted in the amyE locus

This study

 BMB0262

Mutant of strain BMB171 in which the cry2Aa expression cassette is inserted in the amyE locus

This study

 BMB171

Acrystalliferous mutant of B. thuringiensis

[39]

 BMB171Str

Streptomycin spontaneous mutant strain of BMB171, Str^r

This study

 YBT-1520Str

Streptomycin spontaneous mutant strain of B. thuringiensis serovar kurstaki YBT-1520, Str^r

This study

 CT-43

No flagellum, containing cry2Aa expression cassette and thuringiensin synthesis cluster

[40]

 CT-43::Kan

Mutant of strain CT-43 in which the thuE gene is interrupted by the kanamycin-coding gene, also named BMB0545, Kan^r

[41]

Bacillus cereus strains

 ATCC 10987Str

Streptomycin spontaneous mutant strain of B. cereus ATCC 10987, Str^r

This study

 UW85

Wild type B. cereus strain, ZmA producing

[24]

 UW85R

Rifampin spontaneous mutant resistance strain of B. cereus UW85, Rif^r

This study

 BMB0263

Mutant of strain UW85R in which the zmaJ gene is interrupted by in-frame deletion

This study

Escherichia coli strains

 DH5α

F-φ80lacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17 (rk-,mk+) phoA supE44 λ- thi-1 gyrA96 relA1

Invitrogen

Plasmids

 pAW63::Tn5401

A self-transmissible plasmid generated by insertion of the tetracycline-resistance transposon Tn5401 in pAW63, Tet^r

[36]

 pBMB0215

A recombinant plasmid harboring a 4.6-kb HindIII fragment including cry5Ba

[23]

 pBMB0228

17,706-bp endogenous plasmid in strain YBT-1518

[19, 42]

 pBMBmob1

pEMB0603 containing a 1.8-kb BamHI/SphI fragment carrying mob02281

[19]

 pBMBT10

pHT315 containing the recombination cassette with bp 1–352 of oriT1 and bp 1–370 of oriT2

[19]

 pBMBT11

pHT315 containing the recombination cassette with bp 1–352 of oriT1 and bp 156–370 of oriT2

This study

 pBMBT12

pHT315 containing the recombination cassette with bp 1–352 of oriT1 and bp 273–370 of oriT2

This study

 pBMBT13

pHT315 containing the recombination cassette with bp 1–352 of oriT1 and bp 273–297 of oriT2

This study

 pBMBT14

pHT315 containing the recombination cassette with bp 65–282 of oriT1 and bp 1–370 of oriT2

This study

 pBMBT15

pHT315 containing the recombination cassette with bp 188–282 of oriT1 and bp 1–370 of oriT2

This study

 pBMBT16

pHT315 containing the recombination cassette with bp 258–282 of oriT1 and bp 1–370 of oriT2

This study

 pBMBTmini

pHT315 containing the recombination cassette as in pBMBT10 [19] with the 24-bp core sequence of oriT1 and 24-bp core sequence of oriT2

This study

 pEMB0603

E. coli and B. thuringiensis shuttle vector, Amp^r, Kan^r

[35]

 pEMB0603-zmaJ

pEMB0603 containing a 2.1 kb EcoRI/SalI fragment carrying the coding sequence of the seryl-AMP synthetase gene ZmaJ with the promoter of kanamycin resistance gene

This study

 pHT315

E. coli and B. thuringiensis shuttle vector, Amp^r, Erm^r

[43]

 pHT304

E. coli and B. thuringiensis shuttle vector, Amp^r, Erm^r

[43]

 pHT304Ts

E. coli and B. thuringiensis shuttle vector, containing a temperature-sensitive replicon in B. thuringiensis, Amp^r, Erm^r

[35]

 pHTMob02281

pHT304 containing a 1.8-kb HindIII fragment carrying mob02281 and oriT1

This study

 pIC333

An efficient mini-Tnl0 delivery vector in B. subtilis, Spc^r, Erm^r

[33]

 pRec-mob1-Ts

pHT304Ts containing the Mob-Spc cassette and 24-bp core oriT recombination sequence, Amp^r, Erm^r, Spc^r

This study