Fig. 6

Construction of crystal protein gene insertion mutants BMB0261 and BMB0262. Depiction of cry5Ba (a) and cry2Aa (b) insertion. PCR detection with primers amyE-wS and amyE-wA (c) and 5B-S and 5B-A (lanes 1–4) or 2A-S and 2A-A (lanes 5–8) (d); DNA templates were from: 1, pBMB0261 (negative control); 2 and 6, BMB171; 3–4, mutant BMB0261; 5, pBMB0262 (negative control); 7–8, mutant BMB0262; M Trans2 K Plus II DNA marker. e Scanning electron microscopy image of parasporal crystals in unmarked cry integrate mutants. 1, BMB171/cry5Ba-pHT304; 2–3, BMB0261; 4, YBT-1518; 5, BMB171/cry2Aa-pHT304; 6–7, BMB0262; 8, CT-43; 9, BMB171. The arrows indicate the diamond-shaped crystals encoded by cry5Ba and the round-shaped crystals encoded by cry2Aa. Bar indicates 1 μm