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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

Fig. 1

Parasporal crystals and protein expression of cry6Aa in transconjugants. a Phase-contrast microscopy image of parasporal crystals and spores produced in transconjugants. The red arrow indicates the rice-shaped crystals encoded by cry6Aa. The yellow arrow indicates the diamond-shaped crystals encoded by the endogenous crystal genes for YBT1520Str [5] and CT43::Kan [40]. b Protein analysis of the transconjugants by SDS-PAGE. The red arrow indicates the 54-kDa protein produced by cry6Aa. 1 BMB171Str/cry6Aa-pHTMob02281. 2 BMB171Str/pHTMob02281. 3 YBT1520Str/cry6Aa-pHTMob02281. 4 YBT-1520Str/pHTMob02281. 5 CT-43::Kan/cry6Aa-pHTMob02281. 6 CT-43::Kan/pHTMob02281. 7 UW85R/cry6Aa-pHTMob02281. 8 UW85R/pHTMob02281. 9 ATCC 10987Str/cry6Aa-pHTMob02281. 10 ATCC 10987Str/pHTMob02281. M Protein molecular weight marker

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Microbial Cell Factories

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