Table 2 Plasmids used in this study
From: Engineering Escherichia coli for production of 4-hydroxymandelic acid using glucose–xylose mixture
Plasmids | Characteristics | Source |
|---|---|---|
pBldgbrick1 | pMB1 ori with PlacUV5 and Ptrc; ApR | [13] |
pYBT5 | pBldgbrick 1 with P lacUV5 aroG fbr tyrA fbr aroE, P trc ppsA tktA glk | [13] |
pYBH1 | pYSC1 with P lacUV5 T7 RNA polymerase | [13] |
pEBM | pEASY-Blunt with shmaS; pUC ori; KanR | This study |
pHMA | pET28a with shmaS | This study |
pACYCDuet-1 | p15A ori; CmR | Novagen |
pCDFDuet-1 | CloDF13 ori; StrR | Novagen |
pRSFDuet-1 | RSF1030 ori; KanR | Novagen |
pAHM | p15A ori; CmR | This study |
pCHM | CloDF13 ori; StrR | This study |
pRHM | RSF1030 ori; KanR | This study |
pAHM1 | pAHM with Pgap-shmaS | This study |
pAHM2 | pAHM with PlacUV5-shmaS | This study |
pAHM3 | pAHM with Ptrc-shmaS | This study |
pAHM4 | pACYCDuet-1 with PT7-shmaS | This study |
pCHM1 | pCHM with Pgap-shmaS | This study |
pCHM2 | pCHM with PlacUV5-shmaS | This study |
pCHM3 | pCHM with Ptrc-shmaS | This study |
pCHM4 | pCDFDuet-1 with PT7- shmaS | This study |
pRHM1 | pRHM with Pgap-shmaS | This study |
pRHM2 | pRHM with PlacUV5-shmaS | This study |
pRHM3 | pRHM with Ptrc-shmaS | This study |