Fig. 6
Analysis of cellular IgG clearance using Western blot. a Antibody expression in exponentially growing cultures of different strains (wt, Δopi1, Δopi1 + CPR5-PKAR2, wt + CPR5-PGPD and Δopi1 + KAR2-PGAL1 + LHS1-PGAL1) was induced with galactose for 4 h, after which IgG expression was repressed by addition of glucose, in control experiments, the induction was continued. Heavy chain (HC) clearance was followed for 0, 2, 4 and 6 h. After incubations, cell extracts were prepared from the cultures and analyzed using SDS-PAGE and Western blot. A representative blot for tubulin, used as loading control is shown. b Samples from time point zero from all strains were loaded on one gel, and the measured HC signals were visualized using Western blot, their respective tubulin signals are shown as loading controls. c Same as in (a), but samples were reprobed to visualize the light chain (LC) signals. d Same as in (b), but samples were reprobed to visualize the LC signals