Table 4 Plasmids used to compare the activity of CotB3 and CotB4 hydroxylases with different redox system variants
P450 proteins | Redox system | Mutations |
|---|---|---|
CotB3 | AfR/Afx | – |
CotB3 | PdR/Pdx | – |
CotB3 | PdR/Pdx | D38E |
CotB3 | PdR/Pdx | W106E |
CotB3 | PdR/Pdx | D38E, W106E |
CotB4 | AfR/Afx | – |
CotB4 | PdR/Pdx | – |
CotB4 | PdR/Pdx | D38E |
CotB4 | PdR/Pdx | W106E |
CotB4 | PdR/Pdx | D38E, W106E |