Table 2 Plasmids used to construct the cyclooctatin biosynthesis in E. coli Bl21(DE3)
Gene(s) | Vector | Multiple cloning site | Restriction sites |
|---|---|---|---|
crte | pET-Duet-1 | I | NcoI, EcoRI |
cotB2 or txs or cs | pET-Duet-1 | II | NdeI, XhoI |
afR/afx operon or pdR/pdx operon | pACYC-Duet-1 | I | NcoI, NotI |
cotB3/cotB4 operon | pACYC-Duet-1 | II | NdeI, XhoI |