Table 3 Subcellular localization, signal peptide, and prediction of protective antigen for the target proteins
Parameter (program) | SpaC | SodC | NanH | PknG |
|---|---|---|---|---|
Subcellular localization (Psortb) | Cell wall (matched LPXTG; score 9.97) | Cytoplasmic Membrane (matched 61246116: superoxide dismutase Cu–Zn precursor; score 9.68) | Extracellular (matched 585539: sialidase precursor EC 3.2.1.18 NEURAMINIDASE; score 9.70) | Cytoplasmic, (matched 54041713: probable serine/threonine-protein kinase pknG; score 9.89) |
Signal peptide (signalp 4.1)a | No (D = 0.162 D-cutoff = 0.420) | Yes position: 1–35 (cleavage site between pos. 35 and 36: DSA-DK D = 0.631 D-cutoff = 0.450 networks = signalp-TM) | Yes position: 1–31 (cleavage site between pos. 31 and 32: APA-TL D = 0.562 D-cutoff = 0.450 networks = signalp-TM) | No (D = 0.106 D-cutoff = 0.420) |
Prediction of protective antigens (VaxiJen) | Probable ANTIGEN (score 0.6912) | Probable ANTIGEN (score 0.7663) | Probable ANTIGEN (score 0.6967) | Probable NON-ANTIGEN score 0.3686) |