Table 1 Oligonucleotide primers used in this study
From: Cloning, expression and characterization of a β-d-xylosidase from Lactobacillus rossiae DSM 15814T
Primer | Sequence (5′–3′) | Comments |
|---|---|---|
Xyl.a (F) | CGCGGCTAAGATAGGTTCC | Hypotetical protein/Aldoese 1-epimerase (EC 5.1.3.3) forward |
Xyl.a (R) | CTGTCGTGGTCAACGTGTTC | Hypotetical protein/Aldoese 1-epimerase (EC 5.1.3.3) reverse |
Xyl.b (F) | GGAGAACTCGCATGACAATG | β-d-xylosidase (EC 3.2.1.37)/Xyloside transporter (XynT) forward |
Xyl.b (R) | GGTTGTTCATAGCCAGCATAATC | β-d-xylosidase (EC 3.2.1.37)/Xyloside transporter (XynT) reverse |
Xyl.c (F) | GTTGTGTCAGTGGCTGCTG | d-xylose proton symporter (XylT)/Xylose isomerase (EC 5.3.1.5) forward |
Xyl.c (R) | GTGTCAACGATGTAGTGGTTG | d-xylose proton symporter (XylT)/Xylose isomerase (EC 5.3.1.5) reverse |
Xyl.d (F) | GAGTTATGTATTGGGTGTGGAC | Xylulose kinase (EC 2.7.1.17)/Transcriptional regulator forward |
Xyl.d (R) | GAACGCGATGCGTAATAAGAG | Xylulose kinase (EC 2.7.1.17)/Transcriptional regulator reverse |
1108CHisPstF | aaaaaaCTGCAGatgaaaattcaaaatcctgtactg | Restriction site (PstI) flanked by homologous sequence LROS_1108, forward |
1108CHisXbaR | aaaaaaTCTAGACATCACCATCACCATCACttattttgtttctggcaattctttg | Restriction site (XbaI) flanked by 6 x His tag and homologous sequence LROS_1108, reverse |
araRS (F) | GTCTAATGAATCCCTGCTG | Transcriptional regulator ArsR family reverse |
araRS (R) | CCAAAAATCGTGCAGCCG | Transcriptional regulator ArsR family forward |
araR (R) | AACAGTAGCATCAGCAGGTT | Transcriptional repressor 2C Gnt family (araR) reverse |
araR (F) | AGTAAAATGATTGGCGTCAT | Transcriptional repressor 2C Gnt family (araR) forward |
araB (R) | GTAATTGGCGTATTCAAAGC | Ribulokinase (araB) reverse |
araB (F) | CTAGAACAGGTTTGGACTGG | Ribulokinase (araB) forward |
araD (R) | GACTTCGCATTATTTTGACC | l-ribulose-5-phosphate-4-epimerase (araD) reverse |
araD (F) | GAAAAGGGGTTATTCGTCAT | l-ribulose-5-phosphate-4-epimerase (araD) forward |
araA (R) | CGTTGATTTGTTCTTCGTCC | l-arabinose isomerase (araA) reverse |
araA (F) | GTTAGCAGTTCCAGATTACG | l-arabinose isomerase (araA) forward |