Table 3 Oligonucleotides used for polymerase chain reaction
Gene | Oligonucleotide | |
|---|---|---|
Name | Sequence | |
syatprr2 (with His6-tag) | syatprr2_tag_fw | 5′-GGGTTTCATATGAAAGAGACTAACTTCGGCG-3′ |
syatprr2_tag_rev | 5′-CCGCTCGAGTCAGTGGTGATGATGGTGATGACCGCCGACGAAAATTTTCAG-3′ | |
syfiplr (with His6-tag) | syfiplr_tag_fw | 5′-GGGTTTCATATGGGCAAATCCAAAGTTCTG-3′ |
syfiplr_tag_rev | 5′-CCGCTCGAGTCAGTGGTGATGATGG-3′ | |
syfiplr (no tag) | syfiplr_fw | 5′-GGGTTTCATATGGGCAAATCCAAAGTTCTG-3′ |
syfiplr_rev | 5′-CCGCTCGAGTCAAACATAGCGTTTAAGGTATTCTTCAAC-3′ | |