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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis

Fig. 1

The expression level and pattern of GFP were measured in BSG101 and BSG303 (a) and the expression level was analyzed by the SDS-PAGE (b). The spore-deficient BSG1682 was obtained by deleting the σ F which was critical for all spore development in B. subtilis 168. To evaluate strain BSG1682 for the GFP expression, plasmid pBSG03 was transformed into BSG1682 and B. subtilis 168 yielding strains BSG303 and BSG101 respectively. These two strains were cultivated in the same procedure and periodically sampled

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