The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background

Table 3 The major proteins of amyR12 identified by shotgun LC–MS/MS

Protein description	GN	Pep count^a	Unique pepcount^b	Cover percent (%)^c
Glucoamylase	glaA	66	13	38.91
Alpha-amylase A	amyA	61	11	26.65
Probable alpha-galactosidase B	aglB	111	10	27.54
Endoglucanase A	eglA	26	6	26.47
Probable glucan endo-1,3-beta-glucosidase	eglC	19	5	11.96
Aspartic proteinase	pep1	11	5	11.25
Beta-mannosidase A	mndA	101	22	28.03
Mannan endo-1,4-beta-mannosidase	man26A	5	4	13.73
Endo-1,4-beta-xylanase	xynA	2	2	8.23
Endo-1,4-beta-xylanase (fragment)	xynV	32	2	12.76
Beta-xylanase		18	12	35.78

^aThe pepcount refers to the number of total peptides assigned to proteins
^bThe unique pepcount refers to the number of different peptides assigned to the proteins
^cThe percentage coverage is defined as the ratio (%) of the protein sequence covered by the matched peptides

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