Fig. 6
Online measurement data for conventional induction profiling with manual addition of IPTG solution and optical induction profiling with cIPTG. Scattered light and FbFP fluorescence of cultures induced with IPTG (a–d) and cultures induced with cIPTG (e–h). Time of induction and inducer strength (IPTG concentration or duration of UV-A exposure) were varied in full factorial design. Colors from blue to red mark later induction times (0.5–16 h), dull to bright colors mark increasing inducer strength (0–1000 µM IPTG or 0–40 s duration of UV-A exposure). In total, 304 cultures were induced conventionally and 96 cultures were induced optically. The first column (a, c, e, g) shows a subset of cultivations with a fixed induction time of 7.5 h and the second column (b, d, f, h) shows a subset of cultivations with a fixed inducer strength of 400 µM IPTG or 400 µM cIPTG and 40 s UV-A exposure. The online signals for all 400 cultivations are provided in Additional file 9. Small colored down-pointing arrows illustrate the time of induction (not all shown). Long horizontal arrows in black illustrate general trends, e.g. impact of increasing inducer concentration on growth (a). Cultivation conditions: 800 µL Wilms-MOPS mineral medium per well in a 48-FlowerPlate, 400 µM cIPTG added to cultures induced with the LED array (λmax = 368 nm, I = 52 mW/cm2), 30 °C, shaking frequency: 1000 rpm, shaking diameter: 3 mm