Fig. 5

Analysis of cellular response in BALB/c mice immunized with Leishmania-derived particles. Box plot of antigen-specific IFN-γ ELISPOT responses in sHBsAg (right) and 412–425_sHBsAg (left) groups of immunized mice. Splenocytes from six immunized BALB/c mice per group were stimulated with 20 µg/well of 43 different 15-mer overlapping peptides spanning the sHBsAg protein sequence pooled into three groups (P1, P2, P3). The splenocytes were collected 2 weeks after the last immunization. The gray region is the 25–75th percentile; the horizontal lines indicate the mean (dashed) and median (solid) values