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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions

Fig. 6

Out-of-frame translational amyM-gfpmut3 fusion: Plasmid map of pDAamyM-gfp and functional translation of GFP. The plasmid map of pDAamyM-gfp represents a high-copy plasmid for expression of α-amylases carrying a translationally coupled out-of-frame fusion of amyM and gfpmut3. pDAamyM-gfp plasmid ORFs are indicated by thick colored arrows, with the direction of transcription and the identity of each ORF indicated. Relevant restriction sites are also indicated. Sequence details of the amyM-gfpmut3 fusion are shown in the box (a). LCA measurements with B. subtilis 168::pDAamyM-gfp show the functional translation of GFP. The increase of fluorescence intensity over time [dGFP/dT] was monitored using the LCA system with B. subtilis 168 and the 168 strain carrying plasmid pDAamyM-gfp during cultivation in EnPressoB medium (b)

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