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Table 2 Primers used in the present study (restriction sites are underlined)

From: MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

Primer name

Sequence 5′-3′

Kan-U

CGGGGTACCCAGCTGAAGCTTCGTACGC

Kan-D

CGCGGATCCGCATAGGCCACTAGTGGATCTG

MAL62-U

CCGCTCGAGATGACTATTTCTGATCATCC

MAL62-D

CCGCTCGAGTTATTTGACGAGGTAGATT

PGK-U

CGCGGATCCAAGCTTTCTAACTGATCTATCCAAAACTGA

PGK-D

CGCGGATCCAAGCTTTAACGAACGCAGAATTTTC

N-S

ATCATCATCTGTAATCGCTTCACC

K-S

CCTTTTATATTTCTCTACAGGGGCG

N-X

TACAGCGGTAAAGTTTCTATGAGCA

K-X

TAGGTTGTATTGATGTTGGACGAGT

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Microbial Cell Factories

ISSN: 1475-2859

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