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Table 1 Characteristics of strains and plasmids used in the present study

From: MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

Strains or plasmids Relevant characteristic Reference or source
Strains
 Escherichia coli DH5α Φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology
 BY14 MATa/a Industrial baker’s yeast Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology
 BY14aa MATa, haploid derived from BY14 strain This study
 B-NTH1 MATa, ΔNTH1:: loxP This study
 B-NTH1 + K MATa, ΔNTH1:: loxP, Yep-K This study
 BY14a + K MATa, Yep-K This study
 B + MAL62 MATa, Yep-PMK This study
 B-NTH1 + MAL62 MATa, ΔNTH1:: loxP, Yep-PMK This study
Plasmids
 pUG6 E. coli/S. cerevisiae shuttle vector, containing Amp +, loxP-kanMX-loxP disruption cassette [41]
 Yep352 URA3 +, Amp R ori control vector Invitrogen, Carlsbad, Ca, USA
 Yep-K KanMX ARS URA3 +, Amp R ori control vector This study
 pPGK1 bla LEU2 PGK1 P -PGK1 T [42]
 pPGKM bla LEU2 PGK1 P -MAL62-PGK1 T This study
 pUC-ABK NA-loxp-KanMX-loxp-NB Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology
 Yep-PMK bla LEU2 PGK1 P -MAL62-PGK1 T , KanMX This study
 pSH-Zeocin Zeo r, Cre expression vector Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology
  1. aBY14a was selected as high leavening capacity haploid from 32 clones derived from BY14 strain (data not shown)