Table 1 Characteristics of strains and plasmids used in the present study
Strains or plasmids | Relevant characteristic | Reference or source |
|---|---|---|
Strains | ||
Escherichia coli DH5α | Φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 | Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology |
BY14 | MATa/a Industrial baker’s yeast | Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology |
BY14aa | MATa, haploid derived from BY14 strain | This study |
B-NTH1 | MATa, ΔNTH1:: loxP | This study |
B-NTH1 + K | MATa, ΔNTH1:: loxP, Yep-K | This study |
BY14a + K | MATa, Yep-K | This study |
B + MAL62 | MATa, Yep-PMK | This study |
B-NTH1 + MAL62 | MATa, ΔNTH1:: loxP, Yep-PMK | This study |
Plasmids | ||
pUG6 | E. coli/S. cerevisiae shuttle vector, containing Amp +, loxP-kanMX-loxP disruption cassette | [41] |
Yep352 | URA3 +, Amp R ori control vector | Invitrogen, Carlsbad, Ca, USA |
Yep-K | KanMX ARS URA3 +, Amp R ori control vector | This study |
pPGK1 | bla LEU2 PGK1 P -PGK1 T | [42] |
pPGKM | bla LEU2 PGK1 P -MAL62-PGK1 T | This study |
pUC-ABK | NA-loxp-KanMX-loxp-NB | Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology |
Yep-PMK | bla LEU2 PGK1 P -MAL62-PGK1 T , KanMX | This study |
pSH-Zeocin | Zeo r, Cre expression vector | Yeast Collection Center of the Tianjin Key Laboratory of Industrial Microbiology |