Characterisation of the broad substrate specificity 2-keto acid decarboxylase Aro10p of Saccharomyces kudriavzevii and its implication in aroma development

Table 1 Amino acid substitutions in the orthologous enzymes from S. kudriavzevii and S. cerevisiae evaluated by the Grantham score

Name	AAs	Total substitutions		Radical substitutions (≥120)		\(\frac{{\sum {\text{Gr}\text{.}\,\text{score}\,\text{of}\,\text{radicals}} }}{{\sum {\text{Gr}\text{.}\,\text{score}\,\text{total}} }}* 100\,{(\% )}\)
Name	AAs	No.	∑ Grantham score	No.	∑ Grantham score
Permeases
Gap1	602	34	2050	2	275	13.4
Bap2	609	58	3350	6	797	23.8
Bap3	604	45	2276	2	301	13.2
Mup3	546	49	2500	3	478	19.1
Transaminases
Bat1	393	18	817	0	0	0
Bat2	376	41	2640	4	649	24.6
Aro8	500	34	2154	1	180	8.4
Aro9	517	72	3560	3	421	11.8
Decarboxylases
Pdc1	563	10	567	1	125	22.0
Pdc5	563	30	1266	0	0	0
Pdc6	563	NF	–	–	–	–
Aro10	635	87	5764	11	1629	28.3
Dehydrogenases
Adh1	348	15	727	0	0	0
Adh2	348	22	812	0	0	0
Adh3	375	15	821	1	149	18.1
Adh4	382	31	1602	0	0	0
Adh5	351	25	1172	0	0	0
Adh6	360	29	1428	0	0	0
Adh7	361	40	2605	4	584	22.4
Sfa1	385	28	1640	5	700	42.7
Acetyltransferases
Atf1	524	89	5350	6	942	17.6
Atf2	535	110	6187	4	609	9.8
Esterase
Iah1	238	49	2849	3	449	15.8

The corresponding enzymes are involved in the production of aroma-active higher alcohols and acetate esters
NF sequence not found in the S. kudriavzevii database
Radical substitutions involve each substitution with a Grantham score ≥120

ISSN: 1475-2859