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Table 1 Amino acid substitutions in the orthologous enzymes from S. kudriavzevii and S. cerevisiae evaluated by the Grantham score

From: Characterisation of the broad substrate specificity 2-keto acid decarboxylase Aro10p of Saccharomyces kudriavzevii and its implication in aroma development

Name AAs Total substitutions Radical substitutions (≥120) \(\frac{{\sum {\text{Gr}\text{.}\,\text{score}\,\text{of}\,\text{radicals}} }}{{\sum {\text{Gr}\text{.}\,\text{score}\,\text{total}} }}* 100\,{(\% )}\)
No. ∑ Grantham score No. ∑ Grantham score
Permeases
Gap1 602 34 2050 2 275 13.4
Bap2 609 58 3350 6 797 23.8
Bap3 604 45 2276 2 301 13.2
Mup3 546 49 2500 3 478 19.1
Transaminases
Bat1 393 18 817 0 0 0
Bat2 376 41 2640 4 649 24.6
Aro8 500 34 2154 1 180 8.4
Aro9 517 72 3560 3 421 11.8
Decarboxylases
Pdc1 563 10 567 1 125 22.0
Pdc5 563 30 1266 0 0 0
Pdc6 563 NF
Aro10 635 87 5764 11 1629 28.3
Dehydrogenases
Adh1 348 15 727 0 0 0
Adh2 348 22 812 0 0 0
Adh3 375 15 821 1 149 18.1
Adh4 382 31 1602 0 0 0
Adh5 351 25 1172 0 0 0
Adh6 360 29 1428 0 0 0
Adh7 361 40 2605 4 584 22.4
Sfa1 385 28 1640 5 700 42.7
Acetyltransferases
Atf1 524 89 5350 6 942 17.6
Atf2 535 110 6187 4 609 9.8
Esterase
Iah1 238 49 2849 3 449 15.8
  1. The corresponding enzymes are involved in the production of aroma-active higher alcohols and acetate esters
  2. NF sequence not found in the S. kudriavzevii database
  3. Radical substitutions involve each substitution with a Grantham score ≥120