Plasmid | Reference | Size (bp) | Relevant characteristics | Â |
---|---|---|---|---|
pET-30a | Novagen | 5400 | T7 promoter, T7 terminator | Â |
pE194 | [18] | 3728 | Erythromycin resistance gene (ermE) | Â |
pCD256 | [43] | 4790 | Low copy plasmid in L. plantarum | Â |
pCDLbu1 | [15] | 5776 | High copy plasmid in L. plantarum | Â |
pCDLbu1_PT7_mCherry_TT7_Ery | This study | 6425 | T7 RNA polymerase specific promoter | Â |
pCDLbu1ΔEc_PT7_mCherry_TT7_Ery | This study | 3989 | T7 RNA polymerase specific promoter, without sequences for replication and selection in E. coli |  |
pCDLbu1ΔEc_P11_mCherry | [47] | 3809 |  |  |
pCD256_PlacSynth_mCherry | This study | 6959 | Low copy plasmid; promoter PlacSynth; gene of interest mCherry | IPTG (1Â mM) |
pCD256_PlacSynth_T7RNAP | This study | 8882 | Low copy plasmid; promoter PlacSynth; gene of interest T7 RNA polymerase | IPTG (1Â mM) |
pCDLbu1_PlacSynth_mCherry | This study | 8164 | High copy plasmid; promoter PlacSynth; gene of interest mCherry | IPTG (1Â mM), TMG (17Â mM) |
pCDLbu1_PlacA_mCherry | This study | 7917 | High copy plasmid; promoter PlacA; gene of interest mCherry | Lactose (0.5 -2Â % w/v) |
pCDLbu1_PxylA_mCherry | This study | 8077 | High copy plasmid; promoter PxylA; gene of interest mCherry | Xylose (0.2 -2Â % w/v) |
pCDLbu1_PxylA(nativeRBS)_mCherry | This study | 8077 | High copy plasmid; promoter PxylA; gene of interest mCherry; native RBS and spacer sequence from B. megaterium DSMZ 319 xylA | Xylose (2Â % w/v) |
Strains | Â | Â | Â | Â |
B. megaterium DSMZ 319 | DSMZ | Â | Â | Â |
E. coli Neb10β | NEB |  |  |  |
L. plantarum CD033 | [43] | Â | Â | Â |
L. plantarum 3NSH | [17] | Â | Plasmid cured L. plantarum CD033 | Â |