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Table 2 Plasmids and strains used in this study

From: Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum

Plasmid Reference Size (bp) Relevant characteristics  
pET-30a Novagen 5400 T7 promoter, T7 terminator  
pE194 [18] 3728 Erythromycin resistance gene (ermE)  
pCD256 [43] 4790 Low copy plasmid in L. plantarum  
pCDLbu1 [15] 5776 High copy plasmid in L. plantarum  
pCDLbu1_PT7_mCherry_TT7_Ery This study 6425 T7 RNA polymerase specific promoter  
pCDLbu1ΔEc_PT7_mCherry_TT7_Ery This study 3989 T7 RNA polymerase specific promoter, without sequences for replication and selection in E. coli  
pCDLbu1ΔEc_P11_mCherry [47] 3809   
pCD256_PlacSynth_mCherry This study 6959 Low copy plasmid; promoter PlacSynth; gene of interest mCherry IPTG (1 mM)
pCD256_PlacSynth_T7RNAP This study 8882 Low copy plasmid; promoter PlacSynth; gene of interest T7 RNA polymerase IPTG (1 mM)
pCDLbu1_PlacSynth_mCherry This study 8164 High copy plasmid; promoter PlacSynth; gene of interest mCherry IPTG (1 mM), TMG (17 mM)
pCDLbu1_PlacA_mCherry This study 7917 High copy plasmid; promoter PlacA; gene of interest mCherry Lactose (0.5 -2 % w/v)
pCDLbu1_PxylA_mCherry This study 8077 High copy plasmid; promoter PxylA; gene of interest mCherry Xylose (0.2 -2 % w/v)
pCDLbu1_PxylA(nativeRBS)_mCherry This study 8077 High copy plasmid; promoter PxylA; gene of interest mCherry; native RBS and spacer sequence from B. megaterium DSMZ 319 xylA Xylose (2 % w/v)
Strains     
B. megaterium DSMZ 319 DSMZ    
E. coli Neb10β NEB    
L. plantarum CD033 [43]    
L. plantarum 3NSH [17]   Plasmid cured L. plantarum CD033