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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum

Fig. 6

Promoter characteristics and analysis of mCherry expression under control of PT7, regulated by T7 RNA polymerase under control of PlacSynth, and growth behavior. a Change of mCherry expression (RFUs, relative fluorescent units) over time (hours) regulated by T7 RNA polymerase dual plasmid system in BioLector® micro-fermentation is shown. T7 RNAP is under control of PlacSynth and induced with 1 mM IPTG (or non-induced) after 2 h. MRS medium with glucose either 5 μg ml−1 CM or 5 µg ml−1 Ery (or both for the dual plasmid system). –x-: induced; mean values of four replicates are given and standard deviations are indicated. –: non-induced; mean values of three replicates are given and standard deviations are indicated. b Calculated OD600 values. Solid line negative control (n = 3; pCDLbu1ΔEc_PT7_mCherry_TT7_Ery)

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