Fig. 6From: Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum Promoter characteristics and analysis of mCherry expression under control of PT7, regulated by T7 RNA polymerase under control of PlacSynth, and growth behavior. a Change of mCherry expression (RFUs, relative fluorescent units) over time (hours) regulated by T7 RNA polymerase dual plasmid system in BioLector® micro-fermentation is shown. T7 RNAP is under control of PlacSynth and induced with 1 mM IPTG (or non-induced) after 2 h. MRS medium with glucose either 5 μg ml−1 CM or 5 µg ml−1 Ery (or both for the dual plasmid system). –x-: induced; mean values of four replicates are given and standard deviations are indicated. –: non-induced; mean values of three replicates are given and standard deviations are indicated. b Calculated OD600 values. Solid line negative control (n = 3; pCDLbu1ΔEc_PT7_mCherry_TT7_Ery)Back to article page