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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Heterologous production of raspberry ketone in the wine yeast Saccharomyces cerevisiae via pathway engineering and synthetic enzyme fusion

Fig. 3

The Effect of oxygen on the production of raspberry ketone. Strains containing the P. crispum coumarate CoA ligase 2 (Pc4CL2) and benzalacetone synthase (BAS) from R. palmatum (RpBAS) ORFs fused by rigid (r) amino acid linker were fermented in either airlock flasks (anaerobic) or standard flasks (aerobic) in synthetic grape juice medium, supplemented with 3 mM p-coumaric acid and assessed for raspberry ketone production via LC/MS

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