Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production

Table 3 Primers used in this study

Primer	Sequence (5′–3′)
F-as	GCCGGAGCTCATGAATTTACGGCATATTAT
R-as	CGCTCTGCAGTCATGCATTTAATAATTCCG
F-tnaA-FRT	ATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTG
F-tnaA-FRT	AGTGTAGGCTG GAGCTGCTTC
R-tnaA-FRT	TTAAACTTCT TTAAGTTTTG CGGTGAAGTG ACGCAATACT
R-tnaA-FRT	TTCGGTTCGTGTCCATATGA ATATCCTCCT
F-tnaA-check	AAACATCTCCCTGAACCG
R-tnaA-check	GTTTTGCGGTGAAGTGAC
F-sdaA-FRT	GTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTC
F-sdaA-FRT	CGTCTTGAGCGATTGTGTAG
R-sdaA-FRT	TTAGTCACACTGGACTTTGATTGCCAGACCACCGCGTGAGGTTTCGCGGT
R-sdaA-FRT	GATGTAACGCACTGAGAAGC
F-sdaA-check	TGGGGATTGGTCCCTCATCT
R-sdaA-check	GACTTTGATTGCCAGACCACC
F-ggt-FRT	TGGACGCCACTGCCACTCAGGTGGGGGTGGATATTCTCAAGGAGGGCGGG
F-ggt-FRT	CGTCTTGAGCGATTGTGTAG
R-ggt-FRT	AACGCGGCGCATTGGTCGCTTCGGCGACGTTCAAGCCATAATCGATGCTA
R-ggt-FRT	GATGTAACGCACTGAGAAGC
F-ggt-check	TCTGCTCTCAGGAAGTTGTT
R-ggt-check	TAATGCTTTGTGTACTGCCC
F-pepT-FRT	ATGGATAAACTACTTGAGCGATTTTTGAACTACGTGTCTCTGGATACCCA
F-pepT-FRT	CGTCTTGAGCGATTGTGTAG
R-pepT-FRT	AATACGGACGATCACCTGCACCGCTTTTTCCATACCTTCCAGAGTCACAA
R-pepT-FRT	GATGTAACGCACTGAGAAGC
F-pepT-check	TGGATAAACTACTTGAGCGA
R-pepT-check	TTTTCCATACCTTCCAGAGT
F-glgB-FRT	ATGTCCGATCGTATCGATAGAGACGTGATTAACGCGCTAATTGCAGGCCA
F-glgB-FRT	ACGTCTTGAGCGATTGTG
R-glgB-FRT	AGTAAGACGGAGGGCTTGGTCGGTCTATCACCGGTCGCCACCATCGCAGT
R-glgB-FRT	ATGTAACGCACTGAGAAGC
F-glgB-check	TCGTCAACTGGCGTAATCT
R-glgB-check	CAGCAGCGGGTTATCTTT

ISSN: 1475-2859