Microbial Cell Factories

Table 2 Effect of metal ions on EST4 activity

From: A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters

Ion	Relative activity (%)^a
Ion	1 mM	5 mM
Control	100.0 ± 4.2	100.0 ± 0.8
EDTA	97.0 ± 3.6	95.2 ± 2.1
Mg²⁺	95.1 ± 2.9	92.2 ± 0.8
Ca²⁺	94.1 ± 2.9	98.0 ± 1.0
Mn²⁺	92.4 ± 4.7	83.8 ± 3.6
Fe²⁺	87.6 ± 3.3	57.4 ± 1.0
Cu²⁺	87.1 ± 5.3	66.9 ± 4.8
Ni²⁺	78.8 ± 2.5	67.1 ± 2.4
Fe³⁺	77.0 ± 3.0	57.1 ± 4.8
Co²⁺	74.5 ± 4.0	44.5 ± 5.8
Zn²⁺	63.6 ± 3.0	31.6 ± 3.5

^aThe activity toward p-nitrophenyl butyrate without any metal ions was taken as 100 %. All measurements were repeated three times

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ISSN: 1475-2859

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