Effect of cerulenin on fatty acid composition and gene expression pattern of DHA-producing strain Colwellia psychrerythraea strain 34H

Table 1 Effect of cerulenin (12 μg/mL) on the fatty acid composition of C. psychrerythraea 34H

Samples	Fatty acid composition (% of total peak area)
Samples	C10:0	C11:0	C12:0	C12:1^a	C12:1d11	C13:0	C14:0	C14:1^b	C14:1^c	C14:1^d	C15:0	C15:1	C16:0iso	C16:0	C16:1d9t	C16:1d7
	C16:1d9	C17:0	3OH-C12:0	C18:0	C18:1d9t	C18:1d9	C18:1d11	C19:0iso	C22:0	C22:1	C22:3n3	C22:6n3	C31:9
M10	4.3 ± 0.2	0.4 ± 0.0	2.5 ± 0.1	0.3 ± 0.0	0.0 ± 0.0	0.2 ± 0.3	4.1 ± 0.0	0.3 ± 0.0	0.3 ± 0.0	0.0 ± 0.0	2.7 ± 0.0	2.9 ± 0.0	0.2 ± 0.1	28.3 ± 0.9	13.3 ± 0.2	9.0 ± 0.2
M10_C	17.6 ± 0.3	0.4 ± 0.0	5.9 ± 0.1	0.1 ± 0.1	0.0 ± 0.0	0.2 ± 0.3	26.7 ± 0.4	0.3 ± 0.0	0.3 ± 0.0	0.1 ± 0.2	1.1 ± 0.0	0.3 ± 0.2	0.0 ± 0.0	19.7 ± 0.2	0.6 ± 0.0	3.0 ± 0.1
M10	20.6 ± 0.3	0.7 ± 0.1	3.0 ± 0.1	0.7 ± 0.0	0.1 ± 0.1	2.6 ± 0.4	1.2 ± 0.2	0.1 ± 0.1	0.0 ± 0.0	0.0 ± 0.1	0.0 ± 0.0	2.3 ± 0.1	0.2 ± 0.0
M10_C	8.0 ± 0.1	0.0 ± 0.0	3.7 ± 0.2	0.2 ± 0.2	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.3 ± 0.2	1.0 ± 0.1	0.1 ± 0.1	10.1 ± 0.1	0.3 ± 0.3

This strain was cultured at 10 °C for 8 days with or without cerulenin. FAMEs were extracted from two samples and analyzed by GC or GC/MS (See “Methods”). The quantification of each fatty acid from two samples was repeated in triplicate (n = 3). The data was indicated as mean percentage of total peak area ± SEM
^a,b,c,d The double bonds of these fatty acids are still unknown. C16:0iso is branch fatty acid with 15 carbons in main chain, while C19:0iso is branch fatty acid with 18 carbon in main chain. These were confirmed by GC–MS

ISSN: 1475-2859