Fig. 5

Conversion of known CYP1A2 substrates. a HPLC chromatogram of samples from the 7-ethoxyresorufin-O-deethylation assay. Samples were diluted 1:1 with methanol prior to HPLC analysis. (1) Resorufin standard (10 nmol L⁻¹), (2) co-expression sample without substrate in the assay, (3) host cells, (4) cells displaying CPR, (5) cells displaying CYP1A2, (6) cells expressing CYP1A2 and CPR. b Resorufin formation in the whole assay determined by HPLC following expression at 23 or 30 °C. c HPLC chromatogram of samples of the phenacetin-O-deethylation assay. (1) Paracetamol standard (10 µmol L⁻¹) with 8.0 min retention time, (2) 3-acetamidophenol internal standard (10 µmol L⁻¹) with 10.5 min retention time, (3) control sample with co-expression cells without substrate, (4) host cells, (5) cells displaying CPR, (6) cells displaying CYP1A2, (7) cells displaying CYP1A2 and CPR. d MultiCYP activity measured by luciferin formation. Blank: Control sample without cells