Fig. 8

Profiles of conversion of Avicel^® PH-101 or DP by C. glutamicum strains. Celluclast 1.5 L (Sigma; Cat C2730) [75 filter paper unit (FPU)/g-glucan] was used as the cellulolytic enzymes for saccharification of Avicel^® PH-101 (left panels) or DP (right panels). For cellulolytic hydrolysis (upper panels), Avicel (1 % [w/v]) or DP (1 % [w/v]) were hydrolyzed at 30 °C and cellobiose (blue bar) and glucose (red bar) were measured. For SSF (lower panels), Cg-pBbEB1c (black square), Cg-Cello03 (blue triangle) and Cg-Cello04 (red circle) were cultivated with either Avicel (1 % [w/v]) or DP (1 % [w/v]) as sole carbon source in the presence of Celluclast 1.5 L and optical densities at 600 nm were measured after the sedimentation of the residual substrate (lower panels; lines and symbols with left Y-axis). For the measurement of the residual substrate (g/L), each residual substrate was measured at 0 and 24 h from the SSF cultures (lower panels; bars with right Y-axis). During SSF, no cellobiose and glucose were detected in the supernatant from the cultures. Data represents mean values of at least three cultivations and error bars represent standard deviations