Fig. 3
From: Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli
Analysis of antibody fragments produced using CyDisCo in the cytoplasm of E. coli grown in shake flasks. a Coomassie stained non-reducing SDS-PAGE analysis of IMAC purified 3211 (IgG1 mouse) scFv (lane 1) and Fab (lane 2) antibody fragments. b Far UV circular dichroism spectra of IMAC purified 3211 scFv and Fab fragments. c 3211 scFv and Fab binding to recombinant NTproBNP1-76; d Maa48 Fab binding to native and modified LDL; e Maa48 Fab binding to native and modified BSA