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Table 2 Central composite design matrix for experimental and predicted results for catalase activity

From: Tailoring nutritional and process variables for hyperproduction of catalase from a novel isolated bacterium Geobacillus sp. BSS-7

Run Factorsa Experimental results Predicted results
A B C D E F Catalase activity (IU/mg wet weight)
1 0.50 1.00 0.01 6.00 5.00 24.00 42,100 42,200
2 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,400
3 0.50 0.25 0.10 18.00 5.00 12.00 25,600 25,700
4 0.50 0.25 0.01 6.00 5.00 12.00 36,800 35,900
5 1.00 1.00 0.10 12.00 5.00 12.00 46,700 47,400
6 0.50 0.25 0.06 6.00 1.00 24.00 41,900 41,500
7 2.00 1.00 0.01 6.00 1.00 12.00 20,800 20,600
8 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,400
9 2.00 0.25 0.01 18.00 1.00 24.00 40,400 40,300
10 2.00 0.25 0.10 6.00 1.00 12.00 32,000 32,100
11 2.00 0.25 0.01 6.00 5.00 24.00 35,200 35,400
12 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,200
13 2.00 0.25 0.01 18.00 5.00 12.00 41,900 41,600
14 0.50 1.00 0.01 18.00 1.00 24.00 38,500 38,200
15 0.50 0.25 0.10 18.00 1.00 24.00 21,800 20,900
16 0.50 1.25 0.01 18.00 5.00 12.00 23,300 23,100
17 0.50 1.00 0.10 6.00 1.00 12.00 42,100 42,000
18 0.50 0.25 0.10 6.00 10.00 24.00 29,300 29,500
19 2.00 1.00 0.10 6.00 1.00 12.00 39,100 39,200
20 0.50 0.25 0.01 18.00 1.00 12.00 30,300 30,000
21 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,300
22 2.00 1.00 0.10 6.00 1.00 24.00 42,600 42,100
23 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,400
24 2.00 0.25 0.10 18.00 5.00 24.00 38,000 38,100
25 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,200
26 2.00 1.00 0.01 18.00 5.00 24.00 32,000 31,700
27 1.25 1.00 0.10 12.00 3.00 18.00 40,200 40,400
28 1.25 1.00 0.10 12.00 3.00 18.00 40,200 40,400
29 1.25 1.00 0.10 12.00 3.00 18.00 40,200 40,400
30 1.25 1.00 0.10 12.00 3.00 18.00 40,200 40,400
31 1.25 0.63 0.06 12.00 3.00 18.00 19,500 19,700
32 1.25 0.63 0.06 12.00 3.00 8.61 24,800 24,600
33 2.42 0.63 0.06 12.00 3.00 18.00 32,500 32,200
34 1.25 0.04 0.06 12.00 3.00 18.00 9400 9000
35 0.25 1.25 0.06 12.00 3.00 18.00 30,000 28,900
36 1.25 1.00 0.10 12.00 3.00 18.00 40,200 40,400
37 1.25 0.63 0.02 12.00 8.00 18.00 28,200 28,000
38 1.25 0.63 0.06 21.39 3.00 18.00 39,500 40,000
39 1.25 0.63 0.06 12.00 3.00 18.00 11,200 11,500
40 1.25 0.63 0.06 12.00 6.13 18.00 42,100 41,900
41 1.25 0.63 0.13 12.00 8.00 18.00 40,500 39,800
42 1.25 0.63 0.06 12.00 3.00 27.39 24,600 24,700
  1. aSymbols A, B, C, D, E, F are the same as mentioned in Table 5
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Microbial Cell Factories

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