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Table 2 Central composite design matrix for experimental and predicted results for catalase activity

From: Tailoring nutritional and process variables for hyperproduction of catalase from a novel isolated bacterium Geobacillus sp. BSS-7

Run

Factorsa

Experimental results

Predicted results

A

B

C

D

E

F

Catalase activity (IU/mg wet weight)

1

0.50

1.00

0.01

6.00

5.00

24.00

42,100

42,200

2

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,400

3

0.50

0.25

0.10

18.00

5.00

12.00

25,600

25,700

4

0.50

0.25

0.01

6.00

5.00

12.00

36,800

35,900

5

1.00

1.00

0.10

12.00

5.00

12.00

46,700

47,400

6

0.50

0.25

0.06

6.00

1.00

24.00

41,900

41,500

7

2.00

1.00

0.01

6.00

1.00

12.00

20,800

20,600

8

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,400

9

2.00

0.25

0.01

18.00

1.00

24.00

40,400

40,300

10

2.00

0.25

0.10

6.00

1.00

12.00

32,000

32,100

11

2.00

0.25

0.01

6.00

5.00

24.00

35,200

35,400

12

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,200

13

2.00

0.25

0.01

18.00

5.00

12.00

41,900

41,600

14

0.50

1.00

0.01

18.00

1.00

24.00

38,500

38,200

15

0.50

0.25

0.10

18.00

1.00

24.00

21,800

20,900

16

0.50

1.25

0.01

18.00

5.00

12.00

23,300

23,100

17

0.50

1.00

0.10

6.00

1.00

12.00

42,100

42,000

18

0.50

0.25

0.10

6.00

10.00

24.00

29,300

29,500

19

2.00

1.00

0.10

6.00

1.00

12.00

39,100

39,200

20

0.50

0.25

0.01

18.00

1.00

12.00

30,300

30,000

21

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,300

22

2.00

1.00

0.10

6.00

1.00

24.00

42,600

42,100

23

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,400

24

2.00

0.25

0.10

18.00

5.00

24.00

38,000

38,100

25

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,200

26

2.00

1.00

0.01

18.00

5.00

24.00

32,000

31,700

27

1.25

1.00

0.10

12.00

3.00

18.00

40,200

40,400

28

1.25

1.00

0.10

12.00

3.00

18.00

40,200

40,400

29

1.25

1.00

0.10

12.00

3.00

18.00

40,200

40,400

30

1.25

1.00

0.10

12.00

3.00

18.00

40,200

40,400

31

1.25

0.63

0.06

12.00

3.00

18.00

19,500

19,700

32

1.25

0.63

0.06

12.00

3.00

8.61

24,800

24,600

33

2.42

0.63

0.06

12.00

3.00

18.00

32,500

32,200

34

1.25

0.04

0.06

12.00

3.00

18.00

9400

9000

35

0.25

1.25

0.06

12.00

3.00

18.00

30,000

28,900

36

1.25

1.00

0.10

12.00

3.00

18.00

40,200

40,400

37

1.25

0.63

0.02

12.00

8.00

18.00

28,200

28,000

38

1.25

0.63

0.06

21.39

3.00

18.00

39,500

40,000

39

1.25

0.63

0.06

12.00

3.00

18.00

11,200

11,500

40

1.25

0.63

0.06

12.00

6.13

18.00

42,100

41,900

41

1.25

0.63

0.13

12.00

8.00

18.00

40,500

39,800

42

1.25

0.63

0.06

12.00

3.00

27.39

24,600

24,700

  1. aSymbols A, B, C, D, E, F are the same as mentioned in Table 5