Markedly improving asymmetric oxidation of 1-(4-methoxyphenyl) ethanol with Acetobacter sp. CCTCC M209061 cells by adding deep eutectic solvent in a two-phase system

Table 3 Effect of substrate concentration on the asymmetric oxidation of MOPE in [C₄MIM][PF₆]/buffer biphasic system

Substrate concentration (mmol/L)	Initial reaction rate (μmol/min)	Reaction time (h)	Conversion^a (%)	e.e. ^b (%)
55.0	89.2	11.0	51.8	99.9
60.0	93.6	10.5	51.1	99.9
65.0	97.8	10.0	50.5	99.9
70.0	95.9	11.0	49.4	98.6
75.0	84.5	12.0	48.8	96.4
80.0	74.1	13.0	47.5	91.5

Reaction conditions: 4 mL TEA-HCl buffer (100 mmol/L, pH 6.5); 0.3 g/mL immobilized cells; 122 mmol/L acetone; 1 mL [C₄MIM][PF₆]; 55–80 mmol/L MOPE; 30 °C; 220 rpm
^aThe maximum conversion
^bThe residue substrate e.e.

ISSN: 1475-2859